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KMID : 1094720220270030353
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 3 p.353 ~ p.360
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Improvement of Sleeping Beauty Transposon System Enabling Efficient and Stable Protein Production
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Lee Yun-Haeng
Park Ji-Yun
Song Eun-Seon
Lee Ha-Neur
Kuk Myeong-Uk
Joo Jung-Hyun
Roh Hyung-Min
Park Joon-Tae
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Abstract
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The most essential goal in the field of biopharmaceuticals is to develop cell lines with higher protein yields. To this goal, the Sleeping Beauty (SB) transposon-based expression system has been developed as a powerful tool for increasing protein productivity. However, SB transposon system has fallen short of expectation in terms of the efficiency and stability of protein production, limiting its applicability to large-scale production of recombinant proteins. Here, we propose a novel strategy to increase the efficiency and stability of protein production, through modification of the traditional SB transposon vector. Adding a pair of inverted terminal repeats (ITRs) next to existing ITRs (i.e., double-ITRs) significantly increased the efficiency of transgene integration, resulting in high-yield and sustained protein production. Furthermore, double-ITRs responded more favorably to DNA methylation inhibitors in terms of protein yield, implying that using double-ITRs with DNA methylation inhibitors may be effective in increasing protein productivity. Taken together, our study introduces a new vector platform that is applicable to high-yield and sustained protein production, and will open new avenues in the field of biopharmaceuticals.
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KEYWORD
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Sleeping Beauty transposon, double inverted terminal repeats, protein productivity, DNA methylation inhibitor
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